Authors: Monique Juergens Claudia Paetsch Ilona Krämer Marc Zahn Frank Rabenstein Jörg Schondelmaier Edgar Schliephake Rod Snowdon Wolfgang Friedt Frank Ordon
Publish Date: 2009/11/11
Volume: 120, Issue: 4, Pages: 735-744
Abstract
The aphid transmitted Turnip yellows virus TuYV has become a serious pathogen in many rapeseed Brassica napus L growing areas Threeyears’ field trials were carried out to get detailed information on the genetics of TuYV resistance derived from the resynthesised B napus line ‘R54’ and to develop closely linked markers F1 plants and segregating doubledhaploid DH populations derived from crosses to susceptible cultivars were analysed using artificial inoculation with virusbearing aphids followed by DASELISA Assuming a threshold of E 405 = 01 in ELISA carried out in December the results led to the conclusion that prewinter inhibition of TuYV is inherited in a monogenic dominant manner However the virus titre in most resistant lines increased during the growing period indicating that the resistance is incomplete and that the level of the virus titre is influenced by environmental factors Bulkedsegregant marker analysis for this resistance locus identified two closely linked SSR markers along with six closely linked and three cosegregating AFLP markers Two AFLP markers were converted into codominant STS markers facilitating efficient markerbased selection for TuYV resistance Effective markers are particularly valuable with respect to breeding for TuYV resistance because artificial inoculation procedures using virusbearing aphids are extremely difficult to integrate into practical rapeseed breeding programsWe gratefully acknowledge SW Seed GmbH Hadmersleben for providing the DHlines and Norddeutsche Pflanzenzucht NPZ for placing the F1hybrids at our disposal This work was supported by grants of the Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung eV GFP and Arbeitsgemeinschaft industrieller Forschungsvereinigungen “Otto von Guericke” eV AiF
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