Authors: Farida NissanAzzouz Andreas Graner Wolfgang Friedt Frank Ordon
Publish Date: 2004/12/01
Volume: 110, Issue: 2, Pages: 212-218
Abstract
Barley yellow mosaic disease caused by the bymoviruses barley mild mosaic virus BaMMV and barley yellow mosaic virus BaYMV is one of the economically most important diseases of winter barley in Europe In European barley breeding programmes resistance is currently due to only two genes—rym4 which is effective against viruses BaMMV and BaYMV1 and rym5 which is effective against BaYMV2 Diversification of resistance is therefore an important task Because the accession PI1963 confers immunity against all European strains of barley yellow mosaic disease and is not allelic to rym5 we have attempted to develop closely linked markers in order to facilitate the efficient introgression of this resistance into adapted germplasm By means of restriction fragment length polymorphism analysis we located a gene locus for resistance to BaMMV BaYMV1 and BaYMV2 of PI1963 on chromosome 4HL using a mapping population W757 comprising 57 doubled haploid DH lines Subsequent tests for allelism indicated that the BaMMV resistance gene in PI1963 is allelic to rym11 Two DH populations IPK1 and IPK2 comprising 191 and 161 DH lines respectively were derived from the initial mapping population W757 and used for further analysis As random amplified polymorphic DNA development did not facilitate the identification of more closely linked markers simple sequence repeat SSR analyses were conducted For population IPK1 the closest SSRs detected were Bmac181 and Bmag353 which flank the gene at 21 cM and 27 cM respectively For the IPK2 population the SSR markers HVM3 and Bmag353 are located proximally at 25 cM and distally at 82 cM respectively In order to develop markers more tightly linked to rym11 a targeted amplified fragment length polymorphism AFLP marker identification approach was adopted using bulks comprising lines carrying recombination events proximal and distal to the target interval Using this approach we identified six AFLP markers closely linked to rym11 with the two markers E56M32 and E49M33 cosegregating with rym11 in both populations The SSRs and AFLPs identified in this study represent useful tools for markerassisted selectionWe wish to thank Roland Kürschner Annette Plank and Sabine Wagner for their excellent technical assistance Dr Bärbel ForoughiWehr Grünbach/Erding Germany and Dr Heidi Jaiser Pajbjergfonden Denmark for providing DH lines and Dr Frank Rabenstein Federal Centre for Breeding Research on Cultivated Plants for the ELISA antisera
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