Authors: Fahimeh Shahinnia Arnis Druka Jerome Franckowiak Michele Morgante Robbie Waugh Nils Stein
Publish Date: 2011/09/30
Volume: 124, Issue: 2, Pages: 373-384
Abstract
Spike density in barley is under the control of several major genes as documented previously by genetic analysis of a number of morphological mutants One such class of mutants affects the rachis internode length leading to dense or compact spikes and the underlying genes were designated dense spike dsp We previously delimited two introgressed genomic segments on chromosome 3H 21 SNP loci 355 cM and 7H 17 SNP loci 2034 cM in BW265 a BC7F3 nearly isogenic line NIL of cv Bowman as potentially containing the dense spike mutant locus dspar by genotyping 1536 single nucleotide polymorphism SNP markers in both BW265 and its recurrent parent Here the gene was allocated by highresolution biparental mapping to a 037 cM interval between markers SC57808 Hv SPL14–CAPSK06413 residing on the short and long arm at the genetic centromere of chromosome 7H respectively This region putatively contains more than 800 genes as deduced by comparison with the collinear regions of barley rice sorghum and Brachypodium Classical mapbased isolation of the gene dspar thus will be complicated due to the infavorable relationship of genetic to physical distances at the target locusWe gratefully acknowledge Mary Ziems Corine Graser Naser Poursarebani Matthias Jost and Nikki Bonar for excellent technical assistance The project was supported in frame of the ERAPG project BARCODE by grants of SFC Scotland DFG Germany and MUR Italy to RW NS and MM respectively The groups of RW MM and NS collaborate for cereal mutant research in frame of COST action FA0604 Tritigen We thank David Harrap of KWSUK Ltd for making the mutant crosses employed here
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