Authors: Mulualem T Kassa Frank M You Tom G Fetch Pierre Fobert Andrew Sharpe Curtis J Pozniak James G Menzies Mark C Jordan Gavin Humphreys Tingting Zhu MingCheng Luo Curt A McCartney Colin W Hiebert
Publish Date: 2016/04/18
Volume: 129, Issue: 7, Pages: 1373-1382
Abstract
Wheat stem rust caused by Puccinia graminis f sp tritici is a devastating disease of wheat worldwide Development of cultivars with effective resistance has been the primary means to control this disease but the appearance of new virulent strains such as Ug99 has rendered most wheat varieties vulnerable The stem rust resistance gene SrCad located on chromosome arm 6DS has provided excellent resistance to various strains of Ug99 in field nurseries conducted in Njoro Kenya since 2005 Three genetic populations were used to identify SNP markers closely linked to the SrCad locus Of 220 SNP markers evaluated 27 were found to be located within a 2 cM region surrounding SrCad The diagnostic potential of these SNPs was evaluated in a diverse set of 50 wheat lines that were primarily of Canadian origin with known presence or absence of SrCad Three SNP markers tightly linked proximally to SrCad and one SNP that cosegregated with SrCad were completely predictive of the presence or absence of SrCad These markers also differentiated SrCad from Sr42 and SrTmp which are also located in the same region of chromosome arm 6DS These markers should be useful in markerassisted breeding to develop new wheat varieties containing SrCadbased resistance to Ug99 stem rustThe authors thank Leslie Bezte Monika Eng Mira Popovic Ghassan Mardli and Taye Zegeye for excellent technical assistance We are grateful for financial support from Agriculture and AgriFood Canada Growing Forward AAFC Agriflex project 2521 the Canadian Wheat Alliance and as part of CTAG and CTAG2 Genome Prairie projects funded by Genome Canada Saskatchewan Ministry of Agriculture and Western Grain Research Foundation National Research Council contribution NRCC 56190
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