Authors: E Hagihara N Itchoda Y Habu S Iida T Mikami T Kubo
Publish Date: 2005/06/08
Volume: 111, Issue: 2, Pages: 250-255
Abstract
We report here the molecular mapping of a fertility restorer gene named Rf1 for Owen cytoplasmic male sterility in sugar beet Eight AFLP and two RAPD markers tightly linked to the Rf1 locus were identified using bulked segregant analysis Three AFLP markers mAFEM972 mAFEM976 and mAFEM985 were found to cosegregate with the Rf1 allele in our mapping populations With the help of RFLP markers previously mapped on the sugar beet genome we showed that Rf1 is positioned in the terminal region of linkage group Kiel III/Koeln IV This map location agrees well with that found for the restorer gene X which suggests that the Rf1 locus corresponds to the X locus The availability of the molecular markers will facilitate the selection of maintainer–pollinator lines in breeding program and provide the foundation for mapbased cloning of the Rf1 geneThe authors wish to thank Drs C Jung E Barzen and F Salamini for providing RFLP probes A Fujita A Hashimoto and R Takayanagi for technical assistance and the National Agricultural Research Center for Hokkaido Region for sugar beet seeds This work was done in part at the Research Center for Molecular Genetics Hokkaido University and was supported in part by Grants in Aid for Scientific Research from the Ministry of Education Culture Sports Science and Technology Japan and in part by the grant from the Ministry of Agriculture Forestry and Fisheries Japan Research project for utilizing advanced technologies in agriculture forestry and fisheries no 1423 EH was the recipient of the JSPS Fellowship for Japanese Junior Scientists
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