Authors: Xiao Yan Zhong Sinuhe Hahn Vivian Kiefer Wolfgang Holzgreve
Publish Date: 2006/10/06
Volume: 86, Issue: 2, Pages: 139-143
Abstract
Circulatory cellfree DNA cfDNA is increased in a variety of clinical pathologic conditions therefore these markers could be widely used as markers for detecting and monitoring several disorders To better understand the biology of this molecule we analysed the relationship between the level of circulatory cfDNA and physiological parameters such as gender age and frequency of blood donations Paired plasma and serum samples were obtained from 87 blood donors and 50 healthy adults who had never donated blood CfDNA was extracted from plasma and serum samples using the MagNA Pure LC Instrument Quantity determination of circulatory cfDNA was performed by TaqMan realtime PCR for the ubiquitous GAPDH gene Our data showed that the concentration of cfDNA in serum was about eightfold higher than that in plasma Regarding the level of these circulatory species no significant differences were observed between the agematched men and women and gendermatched differentage cohorts except in women who were older than 60 years of age Frequent blood donations did not increase the circulatory species Circulatory cfDNA in plasma and serum samples is not correlated with human gender and human age except in women who are older than 60 years of age Frequent blood donation did not affect the quantity of circulatory cfDNA The explanation for the latter most likely is the short halflife time of free fetal DNA in maternal circulation
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