Authors: Sándor Sipka István Csípő Tamás Czömpöly Péter Balogh Györgyi Vadász Margit Zeher
Publish Date: 2011/01/11
Volume: 90, Issue: 10, Pages: 1227-1228
Abstract
Myeloma proteins arise from the proliferation of an abnormal plasma cell clone generating a homogenous population of monoclonal immunoglobulins These immunoglobulins belong to welldefined classes/subclasses Usually they have normal molecular structures They are biochemically and physicochemically normal immunoglobulins synthesized in an excess amount compared to the physiologic state 1Monoclonal antibodies are functional antibodies for selected antigens One of the best methods for the investigation of antigen specificity is the phage display technology 2 Therefore in the current work we carried out a comparative study on the purified monoclonal antibodies of IgG type from ten patients with multiple myeloma We used ammonium sulfate precipitation combined with DE52 Whatman England gel chromatography for the purification of myeloma IgGs 3 4 The filamentous phage library displaying nine amino acid cyclic random peptide sequences fused to the
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