Authors: Dong Xi XiaoPing Luo QiangHua Lu KaiLun Yao ZuLi Liu Qin Ning
Publish Date: 2007/07/17
Volume: 10, Issue: 3, Pages: 393-400
Abstract
Goldcoated iron oxide nanoparticle Hepatitis B virus HBV DNA probes were prepared and their application for HBV DNA measurement was studied Goldcoated iron oxide nanoparticles were prepared by the citrate reduction of tetrachloroauric acid in the presence of iron oxide nanoparticles which were added as seeds With a fluorescencebased method the maximal surface coverage of hexaethiol 30mer oligonucleotides and the maximal percentage of hybridization strands on goldcoated iron oxide nanoparticles were 120 ± 8 oligonucleotides per nanoparticle and 14 ± 2 respectively which were comparable with those of 132 ± 10 and 22 ± 3 in Au nanoparticle groups Large network aggregates were formed when goldcoated iron oxide nanoparticle HBV DNA gene probe was applied to detect HBV DNA molecules as evidenced by transmission electron microscopy and the high specificity was verified by blot hybridization Our results further suggested that detecting DNA with iron oxide nanoparticles and magnetic separator was feasible and might be an alternative effective methodThis work was supported by the National Science Foundation of China NSFC operating fund 30571643 and 30672380 National Key Basic Research Program of China 2005CB522901 2005CB522507 Clinical Key Program of Ministry of Health of China and National High Technology Program 2002AA302202 2003DF000034 2003CB514112 2004100306805
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