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Title of Journal: J Nanopart Res

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Abbravation: Journal of Nanoparticle Research

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Springer Netherlands

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DOI

10.1007/bf00282004

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1572-896X

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Dispersion quality of amine functionalized multiwa

Authors: Meral Yuce Hikmet Budak
Publish Date: 2014/12/28
Volume: 16, Issue: 12, Pages: 2768-
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Abstract

Impact of dispersion quality of NH2MWCNTs 13–18 nm in diameter with a length between 1 and 12 µm 99  purity in the amplification efficiency of a random DNA oligonucleotide library 96 bp was investigated Amplification yield in the presence of nonfiltered NH2MWCNT dispersion filtered NH2MWCNT dispersion and surfaceattached NH2MWCNTs was explored and physical interactions between NH2MWCNTs and major PCR reagents including DNA template wild type Taq DNA polymerase enzyme and primers were determined using high resolution polyacrylamide gel electrophoresis dynamic light scattering UV–VisNIR spectroscopy and scanning electron microscopy techniques The results revealed that presence of NH2MWCNT dispersion which was sonicated centrifuged and filtered enhanced the total PCR efficiency up to 70  while the presence of NH2MWCNT only centrifuged after sonication inhibited the reaction significantly at similar concentrations Furthermore the NH2MWCNTs coupled covalently onto magnetic microspheres contributed for the specificity enhancement whilst decreasing the amplification efficiency by 30  at the maximum concentration which suggests a removable enhancement system for sensitive applications On the other hand the relative hydrodynamic size distribution measurements displayed a clear difference between the filtered NH2 and nonfiltered NH2MWCNT water dispersions which justifies the inhibition of the amplification by the nonfiltered NH2MWCNTs containing big agglomerates and bundles Finally we demonstrated that major PCR components adsorb onto the NH2MWCNTs with diverse affinities and maintain their functions after adsorption which provides a good framework to further develop tunable NH2MWCNTcarriers to be utilized in various nanobiotechnology and material science applicationsThis work is supported by Scientific and Technological Research Council of Turkey TUBITAK Grant IDs 114Z051 and 113Z611 We would like to acknowledge Hasan Kurt and Ebru Uysal for their expert assistance with SEM and gel electrophoresis respectively

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