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Title of Journal: J Nanopart Res

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Abbravation: Journal of Nanoparticle Research

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Springer Netherlands

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DOI

10.1016/0002-9149(78)90679-3

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1572-896X

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FeSubscript3/SubscriptOSubscript4/Subscript

Authors: Amir Ata Saei Abolfazl Barzegari Mostafa Heidari Majd Davoud Asgari Yadollah Omidi
Publish Date: 2014/07/25
Volume: 16, Issue: 8, Pages: 2521-
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Abstract

Heat shock treatment is the most popular method for transformation of Escherichia coli We have used 19nm Fe3O4 nanoparticles for improving heat shock protocol PGEMT 3000 bp and pCAMBIA 8428 bp were used as test plasmids for transformation of competent E coli cells strains DH5α and Jm107 obtained from heat shock and CaCl2treated bacteria A combination of heat shock and Fe3O4 nanoparticles led to a significant increase 6–10 fold in number of transformed colonies in comparison with heat shock alone The percent increase in transformation efficiency was higher for larger pCAMBIA plasmids compared to PGEMT The transformation efficiency decreased in the absence of CaCl2 and increased by addition of glycerol to the bacterial culture

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