Authors: Kumud Kant Awasthi Anjali Awasthi Narender Kumar Partha Roy Kamlendra Awasthi P J John
Publish Date: 2013/08/07
Volume: 15, Issue: 9, Pages: 1898-
Abstract
Silver nanoparticles Ag NPs are being used increasingly in wound dressings catheters and in various household products due to their antimicrobial activity The present study reports the toxicity evaluation of synthesized and well characterized Ag NPs using Chinese hamster ovary CHO cells The UV–Vis spectroscopy reveals the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 408–410 nm Transmission electron microscopy TEM reveals that the average diameter of silver nanoparticles is about 50 ± 10 nm and that they have spherical shape Cell visibility and cell viability percentage show dosedependent cellular toxicity of Ag NPs The half maximal inhibitory concentration IC50 for CHO cells is 680 ± 265 μg/ml after 24 h Ag NPs exposure Toxicity evaluations including cellular morphology mitochondrial function MTT assay reactive oxygen species ROS and DNA fragmentation assay Ladder pattern were assessed in unexposed CHO cells control and the cells exposed to Ag NPs concentrations of 15 30 and 60 μg/ml for 24 h The findings may assist in the designing of Ag NPs for various applications and provide insights into their toxicityAuthors are grateful to Dr A B Pant Senior Scientist Indian Institute of Toxicological Research Lucknow for providing help in image analysis Authors are thankful to University Grants Commission UGC for financial support One of the authors KA acknowledges the support from Department of Science and Technology DST New Delhi India for Inspire Faculty Program
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