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Title of Journal: J Nanopart Res

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Abbravation: Journal of Nanoparticle Research

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Springer Netherlands

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DOI

10.1002/jwmg.707

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1572-896X

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Anionexchanged nanosolid support of magnetic nano

Authors: T Theppaleak M Rutnakornpituk U Wichai T Vilaivan B Rutnakornpituk
Publish Date: 2013/11/07
Volume: 15, Issue: 12, Pages: 2106-
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Abstract

Poly2diethylaminoethyl methacrylate polyDEAEMAgrafted magnetite nanoparticle MNP with a positively charged surface was used as an anion exchanger for detection of deoxyribonucleic acid DNA sequences by employing peptide nucleic acid PNA as a probe The cationic MNP with a size ranging between 6 and 10 nm in diameter can electrostatically adsorb DNA with a capacity of 171 nmol nucleotide/g MNP The electrostatically neutral pyrrolidinyl PNAbearing prolyl2aminocyclopentane carboxylic acid backbone acpcPNA can be adsorbed by the cationic MNP only when the sequence of the PNA and DNA is complementary and the presence of adsorbed PNA could be examined by matrixassisted laser desorption ionization timeofflight mass spectrometry Two DNA sequences the sequence stimulating Kirsten Rat Sarcoma Kras gene and the sequence having 5′ methylated CpG site were used in this study It was found that the particles can be used as nanosolid support to differentiate between complementary and singlebase mismatched DNA sequences using both single and two acpcPNA probes This polymergrafted MNP might be applicable for use as a magnetically guidable tool for detection of real DNA samples in the future

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