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Title of Journal: J Nanopart Res

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Abbravation: Journal of Nanoparticle Research

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Springer Netherlands

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DOI

10.1016/0022-5088(73)90008-8

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1572-896X

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Avidin conjugation to upconversion phosphor NaYF

Authors: Deyan Kong Zewei Quan Jun Yang Piaoping Yang Chunxia Li Jun Lin
Publish Date: 2008/06/20
Volume: 11, Issue: 4, Pages: 821-829
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Abstract

NaYF4Yb3+ Er3+ nanoparticles were successfully prepared by a polyol process using diethyleneglycol DEG as solvent After being functionalized with SiO2–NH2 layer these NaYF4Yb3+ Er3+ nanoparticles can conjugate with activated avidin molecules activated by the oxidation of the oligosaccharide chain The asformed NaYF4Yb3+ Er3+ nanoparticles NaYF4Yb3+ Er3+ nanoparticles functionalized with amino groups avidin conjugated aminofunctionalized NaYF4Yb3+ Er3+ nanoparticles were characterized by Xray diffraction XRD transmission electron microscopy TEM atomic force microscopy AFM Fourier transform infrared FTIR UV/Vis absorption spectra and upconversion luminescence spectra respectively The biofunctionalization of the NaYF4Yb3+ Er3+ nanoparticles has less effect on their luminescence properties ie they still show the upconversion emission from Er3+ with 4S3/2 → 4I15/2 at ~540 nm and 4F9/2 → 4I15/2 at ~653 nm indicative of the great potential for these NaYF4Yb3+ Er3+ nanoparticles to be used as fluorescence probes for biological system

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