Authors: J Jane Pillow Noah H Hillman Graeme R Polglase Timothy J M Moss Suhas G Kallapur FookChoe Cheah Boris W Kramer Alan H Jobe
Publish Date: 2009/09/15
Volume: 35, Issue: 12, Pages: 2157-2163
Abstract
Lambs delivered at 140 days gestation term = 150 days were assigned to one of five groups n = 5–6/group unventilated controls or ventilation with 21 or 100 O2 that was either heated and humidified or cold and dry Lambs were ventilated gently for 3 h blood gases were recorded regularly Bronchoalveolar lavage and samples of tracheal mucosa and lung were collected post mortemArterial pH was lower mean difference 95 CI −007 −013 −003 while there was an increase in PaCO2 mean difference 95 CI 102 24 179 and fold change in lung proinflammatory IL1β cytokine mRNA mean difference 95 CI 283 03 562 or IL8 mean difference 95 CI 278 79 477 cytokine mRNA expression with 100 O2 relative to 21 O2 Cold dry inspired gas did not influence gas exchange or dynamic mechanics at 3 h compared to heated humidified gas Compared to 100 inspired O2 cold dry inspired gas had less marked effect on fold change in lung proinflammatory IL1β cytokine mRNA mean difference 95 CI 272 −0/8 551 or IL8 mean difference 95 CI 145 55 344 cytokine mRNA expression although cilial dysfunction/damage was evident on electron microscopy especially when exposure to cold dry gas was combined with hyperoxiaWe gratefully acknowledge the technical assistance of Dr Zahra Champion Mr Martin Leckie Dr Ilias Nitsos and Mr Christian Saville in the delivery and care of the animals used in the study This research was supported by HD 12714 from the National Institute of Health USA a NHMRC Career Development Awards JJP 404102 TJMM 303261 and the Women and Infants Research Foundation Ventilator circuits humidifiers and radiant warmer beds used in the studies were provided by Fisher Paykel Healthcare™ New ZealandThis research was supported in part by Fisher Paykel Healthcare™ who market neonatal equipment including ventilator circuits humidifiers and radiant warmer beds Fisher Paykel Healthcare New Zealand as used in the study Representatives from Fisher Paykel Healthcare assisted with care of the newborn lambs under the supervision of Prof Jobe and Prof Pillow Fisher Paykel were not involved in analysis or interpretation of the study measurements or the decision to publish the data They were given the opportunity to read the manuscript prior to submission however their input to the manuscript was limited to comments relating to description of their products
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