Authors: ChiHuei Chiang ChiaoHui Chuang ShiouLing Liu TzongShyuan Lee Yu Ru Kou Haibo Zhang
Publish Date: 2011/05/27
Volume: 37, Issue: 8, Pages: 1360-1367
Abstract
VILI was induced by mechanical ventilation with tidal volume V t of 15 ml/kg in isolated and perfused rat lung Apocynin was administered in the perfusate at onset of mechanical ventilation A group ventilated with low V t of 5 ml/kg served as control Hemodynamics lung injury indices inflammatory responses and activation of apoptotic pathways were determined upon completion of mechanical ventilationThere was an increase in lung permeability and lung weight gain after mechanical ventilation with high V t compared with low V t Levels of inflammatory cytokines including interleukin1β IL1β tumor necrosis factoralpha TNFα and macrophage inflammatory protein2 MIP2 increased in lung lavage fluids concentrations of carbonyl thiobarbituric acid reactive substances and H2O2 were higher in perfusates and lung lavage fluids and expression of myeloperoxidase JNK p38 and caspase3 in lung tissue was greater in the highV t than in the lowV t group Administration of apocynin attenuated these inflammatory responses and lung permeability associated with decreased activation of nuclear factorκBVILI is associated with inflammatory responses including generation of ROS cytokines and activation of mitogenactivated protein kinase cascades Administration of apocynin at onset of mechanical ventilation attenuates inflammatory responses and VILI in the isolated perfused rat lung modelThis work was supported by grants from Taipei Veterans General Hospital VGH 95C1020and VGH98C015 and the National Science Council NSC 95 2314B075008 NSC962314B075006 Taiwan and Canadian Institutes of Health Research MOP77818 and MOP69042 This work was assisted in part by the Division of Experimental Surgery of the Department of Surgery Pathology and Statistic Taipei Veterans General Hospital Taiwan
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