Authors: Pratap Kumar Pati Jaspreet Kaur Pritika Singh
Publish Date: 2010/10/29
Volume: 105, Issue: 3, Pages: 299-307
Abstract
An efficient and cost effective micropropagation protocol using liquid medium was developed for Catharanthus roseus a commercially important medicinal plant Comparative analysis of shoot growth and proliferation in liquid Murashige and Skoog MS medium supplemented with different concentrations of cytokinins 6Benzyladenine BA Kinetin KN and Thidiazuron TDZ was conducted Better response in terms of shoot proliferation shoot diameter number of leaves/shoot number of branches/shoot fresh weight and dry weight was observed in a liquid medium visàvis solid medium A sample of 20 ml of liquid medium supplemented with 5 μM of BA was optimized for propagation of C roseus by a liquid culture system Among various concentrations of auxins tried 1Naphthaleneacetic acid NAA 5 μM was found to be the best for root induction Quantification of pharmaceutically important constituents vincristine and vinblastine and total alkaloid content of microshoots grown in solid and liquid medium as well as in vitro raised plants and mother plant was also conducted hitherto unreported in this highvalue medicinal plant This work further lays the foundations for the shifting of plant production from small to commercial scale
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