Authors: Yue Wang Bernadette van Kronenburg Tila Menzel Chris Maliepaard Xiaohui Shen Frans Krens
Publish Date: 2012/05/25
Volume: 111, Issue: 1, Pages: 113-122
Abstract
To pursue genetic improvement of lily efficiency of both regeneration and transformation from callus cultures induced from different explants were evaluated in multiple cultivars Thirtyfive callus lines induced from filaments or styles and one control callus line derived from bulb scales of in total twenty lily cultivars representing Lilium longiflorum Oriental × Trumpet and Longiflorum × Asiatic hybrids were maintained on a medium with 83 μM picloram PIC In this study they were tested for their regeneration potential by transferring them onto a regeneration medium supplemented with 04 μM PIC and 0044 μM 6benzyladenine Regeneration was obtained in all cultivars examined and the percentage varied from zero to 89 in the 36 callus lines Regeneration frequency was significantly influenced by the genotype cultivar Subculturing the calli every 4 weeks by refreshing the regeneration medium contributed positively to bulblet formation when compared to an eight week subculture frequency It was found that the regeneration ability generally decreased with an increasing age of the callus cultures for all cultivars The origin of the callus style or filament did not lead to significant differences in regeneration frequency but there was an interaction between callus origin and genotype Calli of eight randomly chosen cultivars were cocultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors with the gus gene as reporter and putative transgenic plants were produced GUS histochemical assays demonstrated transient and stable expression of the gus gene in both calli and regenerated lily plants Transient expression frequencies ranged from 03 to 206 while stable transformation was much lower only 14 as the maximum
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