Authors: Jing Li Yang Yu Da Niu Chuan Ping Yang Gui Feng Liu Cheng Hao Li
Publish Date: 2011/02/27
Volume: 106, Issue: 3, Pages: 391-399
Abstract
Somatic embryogenesis SE was induced in female flower buds from mature Schisandra chinensis cultivar ‘Hongzhenzhu’ Somatic embryo structures were induced at a low frequency from unopened female flower buds and excised unopened on Murashige and Skoog MS agar medium containing 40 mg l−1 24dichlorophenoxyacetic acid 24D Friable embryogenic calli were induced from somatic embryo structures after three to four subcultures on initiation medium The frequencies of mature somatic embryo germination and plantlet conversion were low but increased in the presence of gibberellic acid GA3 Some germinated somatic embryos could form friable embryogenic calli on medium without plant growth regulators PGRs The germination and conversion frequencies of somatic embryos from embryogenic calli induced using PGRfree medium were higher than for somatic embryos from embryogenic calli induced on medium containing 24D Most somatic embryos from 24Dinduced embryogenic calli had trumpetshaped embryos and most somatic embryos from PGRfree medium–induced embryogenic calli had two or three cotyledons Histological observation indicated that two and threecotyledon embryos had defined shoot primordia but most of the trumpetshaped embryos yielded plantlets that lacked or had poorly developed meristem tissue Cytological and random amplification of polymorphic DNA RAPD analyses indicated no evidence of genetic variation in the plantlets of somatic embryo originThe authors would like to acknowledge the Fundamental Research Funds for the Central Universities DL09CA12 National Natural Science Foundation of China No 30671701 and Sponsoring the excellent doctor degree dissertation in Northeast Forestry University OPTP10NEFU
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