Authors: Changming Chen Guoju Chen Bihao Cao Jianjun Lei
Publish Date: 2015/06/03
Volume: 122, Issue: 2, Pages: 465-476
Abstract
To identify genes involved in the pollen development and maturation genomewide transcriptional profiling of the flower buds from fertile and sterile plants of the genic male sterile–fertile line 114AB of Capsicum annuum was performed by the RNASeq approach The results indicated that there were numerous changes in gene expression due to the gene mutation in sterile plants A total of 668 differential expressed genes were identified Among them 562 were upregulated and 106 were downregulated genes in the fertile plants compared to those of the sterile plants including 202 and 2 genes specifically accumulated in fertile and sterile plants respectively Hundreds of genes potentially involved in pollen development were identified from the genes specifically expressed in the fertile plants including pollen coat protein lipid binding protein lipid transfer protein pectin methylesterase male sterilityrelated protein antherspecific protein pectate lyase gene and so forth Subsequent analysis of expression patterns for a subset of these genes were verified by semiquantitative RTPCR and quantitative RTPCR Furthermore the upregulated genes were predicted to be associated with intracellular membranebounded organelle cytoplasmic vesicle oxidoreductase activity metal ion binding pollen wall assembly pollen exine formation starch and sucrose metabolism and other processes through Gene ontology and KEGG enrichment analysis Taken together this study provides a list of candidate genes and their expression patterns for pollen development and maturation in pepperThis work was supported by the key project of Guangdong Science and Technology Section 2010A0201020012013B051000069 and Agricultural Section The key project of Guangzhou Science and Technology Section 2011Y20001511BppZXbb3140003 Research Fund for the Doctoral Program of Higher Education 20134404110017 and the Principal Foundation of South China Agricultural University K13012
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