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Title of Journal: Plant Cell Tiss Organ Cult

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Abbravation: Plant Cell, Tissue and Organ Culture (PCTOC)

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Springer Netherlands

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DOI

10.1016/0893-9659(95)00016-j

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ISSN

1573-5044

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Molecular characterization modeling and expressio

Authors: Shonima Talapatra Nirmalya Ghoshal Sarmistha Sen Raychaudhuri
Publish Date: 2013/11/10
Volume: 116, Issue: 3, Pages: 271-283
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Abstract

Somatic embryogenesis receptor kinase SERK gene is known to be a marker of somatic embryogenesis in several plant species The present study reported the isolation and characterization of a SERK gene ortholog designated as McSERK in Momordica charantia an important medicinal plant The complete coding region of McSERK was found to encode a 627 amino acid protein which contained an Nterminal signal peptide a leucine zipper five leucine rich repeats a serineprolineproline domain a transmembrane domain a kinase domain and the Cterminal region depicting the typical characteristic features of SERKfamily proteins Phylogenetic analysis suggested that McSERK was highly similar to the SERK proteins of Cucumis sativus Glycine max and Medicago truncatula Homology modeling was attempted to construct the three dimensional structure of McSERK protein which showed that it corresponded to a monomeric protein McSERK expression was high in embryogenic callus but its expression was relatively low in different plant organs The high expression of McSERK transcript in the embryogenic callus confirmed its association with somatic embryogenesis in M charantia

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