Authors: Selvam Ayarpadikannan Eun Sook Chung Hyun Ah So Kyoung Mi Kim Kenneth Ryan Schraufnagle Jai Heon Lee
Publish Date: 2014/04/09
Volume: 118, Issue: 2, Pages: 327-338
Abstract
Abiotic stresses are the major concern in recent years as their effect on world food production is constantly increasing We have obtained salt tolerant Arabidopsis lines overexpressing SaRBP1 Suaeda asparagoides RNA binding protein 1 of a Korean halophyte S asparagoides Homozygous T3 Arabidopsis transgenic lines were developed and used for salt stress tolerance studies The transgenic seedlings displayed tolerance to salt and mannitol compared to the wild type WT seedlings Transgenic lines produced longer primary roots more fresh weight and higher number of lateral roots than WT In planta stress tolerance assay results showed that the survival rates of transgenic plants were significantly higher than WT plants Transgenic lines showed delayed germination under 200 mM NaCl stress In addition the transgenics showed higher water retention ability than WT Subcellular localization results revealed that SaRBP1 was targeted to the cytoplasm Northwestern blot analysis results confirmed the RNA binding property of SaRBP1 Quantitative RealTime Polymerase Chain Reaction results revealed that many stress marker genes were upregulated by SaRBP1 overexpression Thus our data demonstrate that SaRBP1 overexpression lines are tolerant to salt stress Hence this is the first report for the functional characterization of SaRBP1 a novel RBP gene isolated from S asparagoides cDNA libraryThis research was supported by Basic Science Research Program through the National Research Foundation of Korea KRF funded by the Ministry of Education Science and Technology KRF2012001205 and 2012001273 and the support of Cooperative Research Program for Agriculture Science and Technology Development Project title Isolation and functional analyses of novel genes involved in resistance to abiotic stress for improved soybean productivity Project No PJ007970 Rural Development Administration Republic of Korea
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