Authors: Maria Dolores Arias Padrò Andrea Frattarelli Alessandra Sgueglia Emiliano Condello Carmine Damiano Emilia Caboni
Publish Date: 2011/08/18
Volume: 108, Issue: 1, Pages: 167-172
Abstract
Shoot apices of in vitrogrown plantlets of white mulberry Morus alba L cv Florio were cryopreserved using either encapsulationdehydration or vitrification For encapsulationdehydration alginate beads containing apices were dehydrated for 1 3 5 or 7 days in a liquid medium containing various sucrose concentrations 05 075 10 or 125 M Bead desiccation was performed using silica gel for either 0 4 6 8 9 or 14 h For vitrification apices were directly immersed for either 5 15 30 or 60 min in a vitrification solution PVS2 Following encapsulationdehydration treatment of alginate beads with 075 M sucrose was more effective in promoting regrowth of explants after immersion in liquid nitrogen than in the presence of 05 M sucrose for either 1 or 3 days Regrowth of explants was also observed following vitrification and this reached 47 with increasing duration of the PVS2 treatment from 5 to 30 min Overall the highest frequency of explant regrowth was obtained when explants were subjected to encapsulationdehydration in the presence of 075 M along with a 3 day sucrose dehydration pretreatment and followed by desiccation for 9 h in silica gel
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