Authors: Mariela J Simão Eduardo Fonseca Renata Garcia Elisabeth Mansur Georgia Pacheco
Publish Date: 2015/11/05
Volume: 124, Issue: 2, Pages: 419-430
Abstract
Passiflora pohlii Mast is a wild species native to Brazil with potential agronomic interest Although there are few studies on this particular species recent works described several biological activities in other species of the genus The goal of this work was to establish adventitious roots cultures from stem and root segments excised from in vitrogrown plants of P pohlii as well as to perform phytochemical analyses and evaluate the antioxidant potential of extracts obtained from the in vitro materials in comparison with in vivogrown plants The influence of different parameters type of explant culture systems light and type and concentrations of auxins on the induction of adventitious roots was evaluated Internodal segments showed the best rhizogenesis induction on solidified medium supplemented with 27 µM NAA whereas root segments showed the highest proliferation rate on liquid medium supplemented with 285 µM IAA both in the absence of light TLC analysis indicated the presence of saponins in extracts from all in vivo and in vitroderived materials The antioxidant potential was determined by DPPH and TLCDPPH assays The highest antioxidant activities were observed in extracts from primary and secondary roots of in vivogrown plants The characterization of the phytochemical profile of the in vitro and in vivo materials as well as their pharmacological potential are reported for the first time for this speciesThe authors acknowledge the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro FAPERJ for financial support Mariela Simão is a recipient of a scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES E Mansur is a recipient of a research fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq
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