Authors: Mohd Zaheer Charu Chandra Giri
Publish Date: 2015/05/14
Volume: 122, Issue: 3, Pages: 553-563
Abstract
Multiple shoot induction at an average of 1525 ± 2128 and 1616 ± 3851 shoots/explant was obtained from Andrographis paniculata cotyledonary node explants on Murashige and Skoog’s MS medium supplemented with 20 mg/L 6Benzylaminopurine BAP and Zeatin respectively after 8 weeks of culture Further proliferation of cultures in MS + BAP medium at a maximum of 150 ± 1840 multiple shoots was obtained from single cotyledonary node explants after three subculture 84 days in vitro passage Elicitation as yield enhancement strategy a protocol was developed using multiple shoots to treat the cultures with jasmonic acid JA and salicylic acid SA Various concentrations of JA 1 5 10 25 50 75 and 100 µM and SA 10 20 50 and 100 µM were used for elicitation Treatment of JA at 10 µM concentration resulted in 1322 dry weight DW with 26fold increase in andrographolide production after fifth week compared to 0508 DW in untreated control However JA treatment at 25 and 50 µM promoted 33 30fold enhancement in andrographolide production 1624 and 1481 DW respectively after eighth week compared to control Treatment of 10 20 and 50 µM SA resulted in 30 34 and 31fold andrographolide content 1479 1654 and 1483 DW increase after eighth week respectively compared to control 0478 DW This is the first report on elicitation of A paniculata multiple shoot cultures using signal molecules JA and SA The present findings may be helpful for in vitro manipulation and enhanced production of andrographolidesAuthors would like to thank sponsors University Grants Commission UGC New Delhi OUDSTPURSE project Department of Science and Technology DST New Delhi for financial support Mr Mohd Zaheer thanks UGC and DST OUDSTPURSE New Delhi for the Research Fellowships
Keywords: