Authors: Ertan Sait Kurtar Ahmet Balkaya Dilek Kandemir
Publish Date: 2016/09/01
Volume: 127, Issue: 2, Pages: 497-511
Abstract
The production of large numbers of haploids is the crucial point of the dihaploidisation process Although in vitro haploid plants were successfully produced by irradiated pollen technique in winter squash Cucurbita maxima Duch and pumpkin Cucurbita moschata Duch the frequency is still insufficient for using in a largescale breeding programme Thus the present study was conducted to determine the efficacy of the anther culture technique on the production of in vitro haploids in the aforementioned species for which there have been no successful reports concerning by androgenesis The anthers at uninucleate microspore stage were collected at different florescence times and then cultured on a solid MS medium supplemented by different combinations of 24D 24dichlorophenoxyacetic acid BAP 6benzylaminopurine KN kinetin with the constant addition of NAA naphthalene acetic acid to induce callogenesis embryogenesis and plantlet initiation The combination of PGR genotype and anther collection time played an important role in the androgenic response The highest response was obtained from 57Sİ21 and G9 lines with the combination of 20 or 40 mg/l BAP + 005 mg/l NAA E6 medium at the first anther collection time Plantlets were regenerated and rooted on MS medium supplemented by 001 mg/l IAA In total 74 plants were recovered and propagated with microcuttings The ploidy analyses revealed that 35 plants 473 were haploid n = 20 and the others 527 were diploid 2n = 40
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