Authors: Wei He Bin Guo Penghui Fan Lizhu Guo Yahui Wei
Publish Date: 2014/08/17
Volume: 120, Issue: 1, Pages: 49-55
Abstract
An efficient micropropagation system for Oxytropis glabra a poisonous grass species had been developed Shoot organogenesis occurred from O glabra leaf explants on medium with appropriate supplements of plant growth regulators 856 of shoot regeneration frequency and 82 shoots per leaf explant were achieved when cultured on a medium containing 10 μM 6benzylaminopurine and 10 μM naphthaleneacetic acid Histological studies of these explants confirmed their organogenic nature by demonstrating the formation of shoots Shoot organogenesis was further improved when the leaf explants were preincubated at 4 °C by a 7day pretreatment and shoot regeneration frequency of 884 was recorded with 126 shoots per leaf explant Up to 868 of the regenerated roots formed complete plantlets on a medium containing 10 μM indole3butyric acid within 35 days The micropropagated plants were similar to wild plants Swainsonine was presented in wild O glabra but were not detected in O glabra in vitro culture
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