Authors: Renata Garcia Georgia Pacheco Erica Falcão Gabriela Borges Elisabeth Mansur
Publish Date: 2010/12/19
Volume: 106, Issue: 1, Pages: 47-54
Abstract
Passiflora suberosa is used in popular medicine improvement programs and as an ornamental plant The goal of this study was to establish efficient protocols for plant regeneration and callus induction from nodal internodal and leaf segments excised from in vitrogrown plants The different morphogenetic responses were modulated by the type and concentration of plant growth regulators according to the basal medium and light conditions Shoot formation occurred through three pathways 1 development of preexisting meristems 2 direct organogenesis and 3 indirect organogenesis Development of preexisting meristems was observed from nodal segments 1 shoot/explant in response to αnaphthaleneacetic acid NAA picloram PIC and 24dichlorophenoxyacetic acid 24D using two basal media MS and MSM Direct organogenesis in this species was obtained for the first time in this work through shoot development from internodal segments in the presence of 6benzyladenine BA The highest regeneration rates were achieved on MSM medium regardless of the BA concentration Indirect organogenesis was achieved from all explant types on media supplemented with BA used alone or in combination with NAA The highest regeneration efficiency was obtained from internodal segments cultured on MSM medium plus 444 μM BA Compact friable or mucilaginous nonmorphogenic calluses were induced by thidiazuron PIC 24D and NAA Highyielding friable calluses obtained on MSM medium supplemented with 289 μM PIC are being used for the establishment of suspension cultures and further analysis of the production of bioactive compoundsThe authors acknowledge the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro FAPERJ for a doctoral scholarship and financial support E Mansur is a recipient of a research fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq
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