Authors: Genhai Hu Na Dong Yan Zhou Wuwei Ye Shuxun Yu
Publish Date: 2015/09/12
Volume: 123, Issue: 3, Pages: 673-679
Abstract
Synthetic seeds were obtained in vitro through the encapsulation of axillary buds of Gossypium hirsutum in calcium alginate beads The optimal combination for the production of uniform easy to handle firm and clear beads was found to be 3 sodium alginate in liquid Murashige and Skoog MS medium supplemented with 100 mM CaCl2 The optimal combination for the synthetic endosperm contained 05 mg L−1 indole3butyric acid and 05 mg L−1 αnaphthalene acetic acid which was used in alginate beads in the MS basal medium On this synthetic endosperm medium a 95 synthetic seed germination rate was observed and 358 of synthetic seeds rooted with an average length of 22 cm after 30 days of culturing The optimal synthetic seed coat was obtained using a solution containing 3 sodium alginate and 1 chitosan dissolved in 85 mM acetic acid and solidified liquid containing 200 mM CaCl2 and 002 NaOH Here 961 of synthetic seeds germinated on MS medium and the synthetic seeds were stored in the open for 24 h at 25 °C with only an 8 water loss rate The percentage of seedling emergence from the synthetic seeds was 352 in the aseptic mixture This is the first report on synthetic G hirsutum seeds
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