Authors: Jaanika Edesi Katrin Kotkas Anna Maria Pirttilä Hely Häggman
Publish Date: 2014/07/12
Volume: 119, Issue: 3, Pages: 599-607
Abstract
Cryopreservation the storage of germplasm at ultralow temperature is the most reliable tool for longterm preservation of plant genetic resources Cryopreservation techniques are widely applied but the effect of light spectra on plant recovery after cryopreservation is largely unknown Therefore we investigated the effect of different light spectral qualities on survival and regeneration of shoot tips of potato Solanum tuberosum L cultivars Agrie Dzeltenie Maret Bintje Désirée and Anti cryopreserved by the DMSOdroplet method Prior to cryopreservation the plants were stored under cool white fluorescent light CW Postcryopreservation the plants were allowed to regenerate under six different light qualities CW warm white light HQI blue LEDs B red LEDs R red with 10 of blue RB and RBF red with 10 of blue with addition of 20 of farred LEDs The light spectral quality had a significant effect on the survival and regeneration of potato shoot tips after cryopreservation The combination of red light with 10 of blue RB doubled the regeneration percentage of all cultivars whereas red light R was not suitable for regeneration after cryopreservation Specifically the regeneration percentages were increased in RB compared to CW from 255 to 526 for ‘Agrie Dzeltenie’ 250–436 for ‘Maret’ 81–261 for ‘Bintje’ 00–171 for ‘Anti’ and 182–366 for ‘Désirée‘ Therefore the modification of light spectra during the recovery phase is a promising tool for increasing the regeneration of potato shoot tips after cryopreservation
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