Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Chem Nat Compd

Search In Journal Title:

Abbravation: Chemistry of Natural Compounds

Search In Journal Abbravation:

Publisher

Kluwer Academic Publishers-Plenum Publishers

Search In Publisher:

DOI

10.1007/s10529-008-9634-4

Search In DOI:

ISSN

1573-8388

Search In ISSN:
Search In Title Of Papers:

Protease A from cotton seeds Isolation and purifi

Authors: L G Mezhlumyan M A Kuchenkova P Kh Yuldashev
Publish Date: 1986/11/01
Volume: 22, Issue: 6, Pages: 686-689
PDF Link

Abstract

Protease A has been isolated in the homogeneous state from dormant seeds of cotton plants of the TashkentI variety A scheme is proposed for the isolation and purification of the enzyme which includes the following stages extraction of the defatted seeds with 01 M phosphate buffer pH 74 precipitation of the protein with ammonium sulfate at 60 saturation desalting by dialysis and ionexchange chromatography on a column containing CM and DEAEcelluloses The molecular weight of the enzyme has been determined as 60000 The enzyme efficiently hydrolyzes azocasein and the 7S and 11S reserve proteins of cotton seeds Its maximum activity appears at pH 64–74 and a temperature of 35–40°C it is not activated by sulfhydryl reagents and loses its activity in the presence of diisopropyl phosphorofluoridate The assumption is made that protease A belongs to the serine type of trypsinlike proteases

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Indole alkaloids from the roots of Isatis ingigotica and their antiherpes simplex virus type 2 (HSV-2) activity in vitro
  2. A study of the properties and 13 C NMR spectra of pinifolic acid and its derivatives
  3. Quinoid pigments of echinodermata V. Pigments of the sea urchin Strongy locentrotus dröebachiensis
  4. Composition of the essential oil of Larix sibirica
  5. A New Cyclic Peptide from Bulbs of Fritillaria hupehensis
  6. Automation of the optimization of the separation of protamines by high-performance liquid chromatography
  7. Chemical synthesis and enzymatic assembly of fragments of the DNA coding immunodominant epitopes of human immunodeficiency virus
  8. Extraction of the total alkaloids from the epigeal part of Aconitum orientale
  9. Use of the method of metastable defocusing for the analysis of the processes involved in the fragmentation of lycoctonine bases
  10. Chemical Constituents of Cinnamomum rupestre and Cinnamomum nanophyllum
  11. The use of the intramolecular nuclear Overhauser effect to establish the positions of substituents in the aromatic nuclei of the alkaloids majoridine and O-acetyl-N-methyllochnerine
  12. Chemical composition of the essential oils of mongolian wormwoods Artemisia xerophytica and A. xantaphora
  13. Effect of polyhydroxysteroids from starfish and brittle stars on the activity of β-1,3-glucanases
  14. Evaluation of vinasses from sugarcane molasses distillation as a new source of sugarcane wax
  15. Phenolic Compounds from Roots of Lupinus polyphyllus
  16. Preparation of (2′-5′)-oligonucleotides based on 6- N -benzylaminopurineriboside using the Spicaria violacea fungal enzyme complex
  17. Rapid isocratic HPLC analysis of caffeic acid derivatives from Echinacea purpurea cultivated in Iran
  18. Isolation and Identification of Radical Scavenging Components of Seeds of Desmodium styracifolium
  19. Synthesis of actiphenol and its normethyl analogues and 6-hydroxy derivatives
  20. General characteristics of immunomodulator bioglycans from invertebrates of the sea of Japan
  21. Modifying Effect of Betulinol on Carbon Sorbents Produced from Betula birch Wood
  22. A new arylsulfatase from the marine mollusk Turbo chrysostomus
  23. Isolation and identification of furanocoumarins and a phenylpropanoid from the acetone extract and identification of volatile constituents from the essential oil of Peucedanum pastinacifolium
  24. A New Diterpene Isolated from the Resin of Hymenaea Courbaril
  25. DNA-protector and cytotoxic activity of secoisolariciresinol diglucoside derivatives
  26. Biological and phytochemical investigations on Ajania fruticulosa
  27. Two new ceramides from Zephyranthes candida
  28. Glycosylation of cardenolides. VIII. Structures of the by-products of the synthesis by the orthoester method of strophanthidol rhamnosides
  29. Avarol and isoavarol from a Pacific Ocean sponge Dysidea sp.
  30. Synthetic and modified isoflavonoids. II. Synthesis of new 2,6-dialkyl-substituted analogues of pseudobaptigenin
  31. Composition of the essential oil of Adiantum flabellulatum
  32. Diacetylisostachyflaside and acetylisostachyflaside from Stachys atherocalyx
  33. Berberis alkaloids XXXII. Berberal — A new alkaloid from Berberis heterobotrys
  34. New oligomeric proanthocyanidin glycosides platanoside-A and platanoside-B from Platanus orientalis trunk bark
  35. Chemical investigation of Hippophaë rhamnoides . I. The main components of the unsaponifiable part of an extract of the fruit pulp
  36. Chemical investigation of Hippophaë rhamnoides . I. The main components of the unsaponifiable part of an extract of the fruit pulp
  37. A new butanolide from the leaves of Cinnamomum reticulatum
  38. Some Biological Compounds, Antioxidant and Antimicrobial Activities of Endemic Hypericum uniglandulosum
  39. Chemical constituents from Verbena officinalis
  40. Conjugates of Polyguluronic and Polymannuronic Acids with 4-Aminoantipyrine. Determination Using 1 H– 13 C HSQC Spectra of Triad Units and Their Distribution in the Polysaccharide Chain
  41. Synthesis and anti-HIV activity of triterpene 3- O -galactopyranosides, analogs of glycyrrhizic acid
  42. Structure of the alkaloids of Aerva lanata
  43. Insect pheromones and their analogs. VIII. Synthesis of the (Z) and (E) isomers of 2-methyloctadec-7-ene and of 2-methyl-7,8-epoxyoctadecane
  44. Isolation and study of proteinases from the milky juice of Carica papaya grown under hothouse conditions in Uzbekistan
  45. Compactin — A new orobol bioside from Genista compacta
  46. Cyclization of cembrane diterpenoids. VI. Products of the cyclization of 5β-acetoxyisocembrol
  47. Fatty acid composition of Prosopis cineraria seeds
  48. Proteins responsible for seed pubescence of cotton genetic lines
  49. Products of the ozonolysis of epitorulosol
  50. Isolation, Synthesis, and Antifungal Activity of Kojic Acid and its Derivatives
  51. Synthesis and biological activity of aminoacyl phenothiazine derivatives based on the alkaloids cytisine, anabasine, and d -pseudoephedrine
  52. Secondary metabolites from Leucaena leucocephala
  53. New Mucic Acid Gallates from Phyllanthus emblica
  54. Dimeric tropane alkaloids
  55. Flavonoids and anthocyans from Alhagi pseudoalhagi
  56. Volatiles in flowers of Viburnum odoratissimum
  57. Terpenoids of Pinus funebris
  58. Fatty Acid and Metal Composition of the Seeds of Vicia ervilia Varieties from Turkey
  59. Volatile Constituents of Senecio tenuifolius From India
  60. Natural Dibenzo[ b , d ]Pyran-6-Ones: Structural Diversity and Biological Activity
  61. Determination of the Volatile Composition of Tessaratoma papillosa NYMPH by GC/MS
  62. Composition of cottonseed soapstock fats
  63. Unusual Meroterpenoids from Leaves of Psidium guajava
  64. Biochemical characterization of the vital activity of Yersinia pseudotuberculosis in a culture medium as a function of the temperature of cultivation
  65. New Oxirapentyn E from Marine Isolate of the Fungus Isaria felina
  66. Chemical Constituents and Antibacterial Activity of the Essential Oil of a Marine-Derived Fungus from the South China Sea
  67. Alkaloids from the Rhizomes of Iris germanica
  68. Interaction of (13S)-6-oxolabd-7,14-dien-13-ol with phenols on the clay askanite-bentonite
  69. Alkaloids of Veratrum lobelianum the structure of germinaline
  70. Chemical constituents of the stem bark of Fraxinus rhynchophylla
  71. Oxygenated fatty acids of the seeds of Elaeagnus angustifolia
  72. Sesquiterpenoids from Aster himalaicus
  73. Alkaloids of Corydalis gortschakovii
  74. Coumarins of Ferulago turcomanica
  75. Dynamics of the accumulation of the flavan compounds of Hibiscus cannabinus
  76. Chemical Constituents of the Roots of Zizyphus rugosa
Search Result: