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Title of Journal: Chem Nat Compd

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Abbravation: Chemistry of Natural Compounds

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Springer US

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DOI

10.1007/bf01776130

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1573-8388

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Phenolic Compounds from Roots of Emphasis Type="I

Authors: V V Boinik Kh P Akritidu O V Demeshko
Publish Date: 2015/03/27
Volume: 51, Issue: 2, Pages: 352-352
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Abstract

The investigation used reversedphase RP chromatography over a Supelco Discovery C18 chromatography column 250 × 46 mm with silica gel 5 μm modified by octadecyl groups The mobile phase was solvent A 01 trifluoroacetic acid TFA 5 MeCN H2O pH 208 and solvent B 01 TFA MeCN The chromatography conditions were mobilephase flow rate 01 mL/min eluent operating pressure 400 bar 40 kPa column thermostat temperature 25°C sample volume 10 μL and chromatography time 40 min We used gradient elution The scan time was 06 s at wavelengths 280 and 255 nmGround raw material 20 g accurate weight was placed into a 100mL flask and extracted with refluxing doubly distilled H2O 50 mL for 30 min on a boilingwater bath The obtained extract was filtered cooled transferred to a 100mL flask and adjusted to the mark with doubly distilled H2O 2

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