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Title of Journal: Chem Nat Compd

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Abbravation: Chemistry of Natural Compounds

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Kluwer Academic Publishers-Plenum Publishers

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DOI

10.1002/pssb.2221080240

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1573-8388

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Chemical synthesis and enzymatic assembly of fragm

Authors: M G Isagulyants S V Luchin V D Smirnov
Publish Date: 1990/05/01
Volume: 26, Issue: 3, Pages: 328-332
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Abstract

More than twenty 13 to 55membered oligopolynucleotides have been synthesized by the Hphosphonate solidphase method in the manual variant using an original procedure From the oligonucleotides obtained seven DNA duplexes coding immunodominant epitopes of HIV1 proteins have been formed 598–609 and 737–748 gp41 91–115 and 105–115 gag and 940–951 pol and a number of its mutants The ligase assembly and polymerization of the DNA duplexes obtained has been carried out The efficiency of ligation amounted to from 60 to 90 The possibility of their directed polymerization was determined by the flanking of the duplexes by partially completed halfsites of restrictases BamHI and XhoI

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Other Papers In This Journal:
  1. Indole alkaloids from the roots of Isatis ingigotica and their antiherpes simplex virus type 2 (HSV-2) activity in vitro
  2. A study of the properties and 13 C NMR spectra of pinifolic acid and its derivatives
  3. Quinoid pigments of echinodermata V. Pigments of the sea urchin Strongy locentrotus dröebachiensis
  4. Composition of the essential oil of Larix sibirica
  5. A New Cyclic Peptide from Bulbs of Fritillaria hupehensis
  6. Automation of the optimization of the separation of protamines by high-performance liquid chromatography
  7. Extraction of the total alkaloids from the epigeal part of Aconitum orientale
  8. Use of the method of metastable defocusing for the analysis of the processes involved in the fragmentation of lycoctonine bases
  9. Chemical Constituents of Cinnamomum rupestre and Cinnamomum nanophyllum
  10. The use of the intramolecular nuclear Overhauser effect to establish the positions of substituents in the aromatic nuclei of the alkaloids majoridine and O-acetyl-N-methyllochnerine
  11. Chemical composition of the essential oils of mongolian wormwoods Artemisia xerophytica and A. xantaphora
  12. Effect of polyhydroxysteroids from starfish and brittle stars on the activity of β-1,3-glucanases
  13. Evaluation of vinasses from sugarcane molasses distillation as a new source of sugarcane wax
  14. Phenolic Compounds from Roots of Lupinus polyphyllus
  15. Preparation of (2′-5′)-oligonucleotides based on 6- N -benzylaminopurineriboside using the Spicaria violacea fungal enzyme complex
  16. Rapid isocratic HPLC analysis of caffeic acid derivatives from Echinacea purpurea cultivated in Iran
  17. Isolation and Identification of Radical Scavenging Components of Seeds of Desmodium styracifolium
  18. Synthesis of actiphenol and its normethyl analogues and 6-hydroxy derivatives
  19. General characteristics of immunomodulator bioglycans from invertebrates of the sea of Japan
  20. Modifying Effect of Betulinol on Carbon Sorbents Produced from Betula birch Wood
  21. A new arylsulfatase from the marine mollusk Turbo chrysostomus
  22. Isolation and identification of furanocoumarins and a phenylpropanoid from the acetone extract and identification of volatile constituents from the essential oil of Peucedanum pastinacifolium
  23. A New Diterpene Isolated from the Resin of Hymenaea Courbaril
  24. DNA-protector and cytotoxic activity of secoisolariciresinol diglucoside derivatives
  25. Biological and phytochemical investigations on Ajania fruticulosa
  26. Two new ceramides from Zephyranthes candida
  27. Glycosylation of cardenolides. VIII. Structures of the by-products of the synthesis by the orthoester method of strophanthidol rhamnosides
  28. Avarol and isoavarol from a Pacific Ocean sponge Dysidea sp.
  29. Protease A from cotton seeds. Isolation and purification of the enzyme
  30. Synthetic and modified isoflavonoids. II. Synthesis of new 2,6-dialkyl-substituted analogues of pseudobaptigenin
  31. Composition of the essential oil of Adiantum flabellulatum
  32. Diacetylisostachyflaside and acetylisostachyflaside from Stachys atherocalyx
  33. Berberis alkaloids XXXII. Berberal — A new alkaloid from Berberis heterobotrys
  34. New oligomeric proanthocyanidin glycosides platanoside-A and platanoside-B from Platanus orientalis trunk bark
  35. Chemical investigation of Hippophaë rhamnoides . I. The main components of the unsaponifiable part of an extract of the fruit pulp
  36. Chemical investigation of Hippophaë rhamnoides . I. The main components of the unsaponifiable part of an extract of the fruit pulp
  37. A new butanolide from the leaves of Cinnamomum reticulatum
  38. Some Biological Compounds, Antioxidant and Antimicrobial Activities of Endemic Hypericum uniglandulosum
  39. Chemical constituents from Verbena officinalis
  40. Conjugates of Polyguluronic and Polymannuronic Acids with 4-Aminoantipyrine. Determination Using 1 H– 13 C HSQC Spectra of Triad Units and Their Distribution in the Polysaccharide Chain
  41. Synthesis and anti-HIV activity of triterpene 3- O -galactopyranosides, analogs of glycyrrhizic acid
  42. Structure of the alkaloids of Aerva lanata
  43. Insect pheromones and their analogs. VIII. Synthesis of the (Z) and (E) isomers of 2-methyloctadec-7-ene and of 2-methyl-7,8-epoxyoctadecane
  44. Isolation and study of proteinases from the milky juice of Carica papaya grown under hothouse conditions in Uzbekistan
  45. Compactin — A new orobol bioside from Genista compacta
  46. Cyclization of cembrane diterpenoids. VI. Products of the cyclization of 5β-acetoxyisocembrol
  47. Fatty acid composition of Prosopis cineraria seeds
  48. Proteins responsible for seed pubescence of cotton genetic lines
  49. Products of the ozonolysis of epitorulosol
  50. Isolation, Synthesis, and Antifungal Activity of Kojic Acid and its Derivatives
  51. Synthesis and biological activity of aminoacyl phenothiazine derivatives based on the alkaloids cytisine, anabasine, and d -pseudoephedrine
  52. Secondary metabolites from Leucaena leucocephala
  53. New Mucic Acid Gallates from Phyllanthus emblica
  54. Dimeric tropane alkaloids
  55. Flavonoids and anthocyans from Alhagi pseudoalhagi
  56. Volatiles in flowers of Viburnum odoratissimum
  57. Terpenoids of Pinus funebris
  58. Fatty Acid and Metal Composition of the Seeds of Vicia ervilia Varieties from Turkey
  59. Volatile Constituents of Senecio tenuifolius From India
  60. Natural Dibenzo[ b , d ]Pyran-6-Ones: Structural Diversity and Biological Activity
  61. Determination of the Volatile Composition of Tessaratoma papillosa NYMPH by GC/MS
  62. Composition of cottonseed soapstock fats
  63. Unusual Meroterpenoids from Leaves of Psidium guajava
  64. Biochemical characterization of the vital activity of Yersinia pseudotuberculosis in a culture medium as a function of the temperature of cultivation
  65. New Oxirapentyn E from Marine Isolate of the Fungus Isaria felina
  66. Chemical Constituents and Antibacterial Activity of the Essential Oil of a Marine-Derived Fungus from the South China Sea
  67. Alkaloids from the Rhizomes of Iris germanica
  68. Interaction of (13S)-6-oxolabd-7,14-dien-13-ol with phenols on the clay askanite-bentonite
  69. Alkaloids of Veratrum lobelianum the structure of germinaline
  70. Chemical constituents of the stem bark of Fraxinus rhynchophylla
  71. Oxygenated fatty acids of the seeds of Elaeagnus angustifolia
  72. Sesquiterpenoids from Aster himalaicus
  73. Alkaloids of Corydalis gortschakovii
  74. Coumarins of Ferulago turcomanica
  75. Dynamics of the accumulation of the flavan compounds of Hibiscus cannabinus
  76. Chemical Constituents of the Roots of Zizyphus rugosa
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