Authors: Alberto MoralesVillagrán Cuauhtemoc SandovalSalazar Laura MedinaCeja
Publish Date: 2008/04/22
Volume: 33, Issue: 8, Pages: 1592-1598
Abstract
Glutamate Glu is the main excitatory neurotransmitter in the brain for which several methods have been developed to measure this compound in extracellular brain fluids Most of these techniques are based on coupling microdialysis to HPLC and they have a resolution time of about 10 min Here we present a different approach to measure Glu with a resolution of about 1 min per microdialysis sample enabling a better relationship to be established between EEG activity and biochemical changes This new setup was used to determine the time delay between the tip of the microdialysis probe and the site of sample collection and was accurate to within seconds Indeed the measurement of Glu concentrations was linear Administration of 4aminopyridine was used to provoke seizure convulsions and under these conditions biochemical changes and EEG activity were evaluated These experimental data support the key role of Glu in the initiation of a seizure convulsionWe want to thank Dr Ricardo Tapia for offering us the benefit of his experience and knowledge in this field and for allowing us to work with him We also wish to thank Dr Clorinda Arias for coordinating this work and Veronica PalomeraAvalos for her technical assistance This work was supported by CONACYT Project No 48002
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