Authors: Jiane Liu Yuqing Shen Mingli Li Qian Shi Aifeng Zhang Fengqin Miao Junhua Liu Xiaojing Wu Youji He Jianqiong Zhang
Publish Date: 2012/11/17
Volume: 38, Issue: 2, Pages: 290-299
Abstract
Classical major histocompatibility complex MHC class I first identified in the immune system is also expressed in the developing and adult central nervous system CNS Although the MHC class I molecules have been found to be expressed in the CNS of different species a necessary step to elucidate the temporal and spatial expression patterns of MHC class I molecules in the brain development has never been taken Frozen sections were made from the brains of embryonic and postnatal C57BL/6 J mice and the expression of H2Db mRNA was examined by in situ hybridization Immunofluorescence was also performed to define the cell types that express H2Db in P15 mice At E105 the earliest stage we examined H2Db was expressed in neuroepithelium of the brain vesicles From E125 to P0 H2Db expression was mainly located at cerebral cortex neuroepithelium of the lateral ventricle neuroepithelium of aquaeductus and developing cerebellum From P4 to adult H2Db mRNA was detected at olfactory bulb hippocampus cerebellum and some nerve nuclei The major cell types expressing H2Db in P15 hippocampus cerebral cortex and olfactory bulb were neuron H2Kb signal paralleled that of H2Db and the expression levels of the two molecules were comparable throughout the brain The investigation of the expression pattern of H2Db at both embryonic and postnatal stages is important for further understanding the physiological and pathological roles of H2Db in the developing CNS
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