Authors: Małgorzata Beręsewicz Joanna E Kowalczyk Barbara Zabłocka
Publish Date: 2008/03/13
Volume: 33, Issue: 9, Pages: 1789-1794
Abstract
Regulators of mitogen activated protein kinases MAPK and cJun Nterminal/stressactivated kinase JNK include Rholike small GTPbinding proteins and their regulators SynGAP and kalirin7 are postsynaptic densityenriched proteins identified through their interaction with Rho GTPases and PSD95 scaffold protein We examined immunoreactivity of SynGAP kalirin7 and PSD95 phosphorylation of MAPK and JNK in control and postischemic hippocampus in gerbil model of transient forebrain ischemia In normal brain higher amount of kalirin7 but a lower amount of PJNK was found in ischemiaresistant hippocampal area CA23 DG than in ischemiavulnerable CA1 After 5 min ischemia and 1 h reperfusion a decrease of PERK and increase of PJNK were uniformly observed in the hippocampal parts By contrast the amount of kalirin7 in CA23 DG reached 56 P 0001 of control while was doubled in CA1 Oppositely the immunoreactivity of SynGAP was increased in CA23 DG and reduced in CA1 Our data indicate that SynGAP and kalirin7 take part in the regulation of ischemic signal transduction but the mechanism does not seem directly connected with the activation of MAPK and JNK
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