Journal Title
Title of Journal: Neurochem Res
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Abbravation: Neurochemical Research
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Authors: Yugang Liu Jian Zheng Yan Zhang Zhaotao Wang Yang Yang Miaochun Bai Yiwu Dai
Publish Date: 2016/07/09
Volume: 41, Issue: 10, Pages: 2728-2751
Abstract
Fucoxanthin is rich in seaweed and considered as effective anticancer drug because of powerful antioxidant properties The objective of this study was to investigate the role of fucoxanthin on apoptosis invasion and migration of glioma cells Firstly fucoxanthin showed obvious cytotoxicity against human glioma cancer cell line U87 and U251 however there was no inhibitory effect on normal neuron And then fucoxanthin induced apoptotic cell death showed by the condensation of chromatin material stained with Hoechest 33342 and reduced mitochondrial membrane potential via DIOC63 staining and enhanced apoptosis by annexin VFITC/SYTOX Green double staining on U87 and U251 cell lines Transmission electron microscopy and western blotting were used to determine ultrastructure of U87 cell and expression of proteins related to apoptosis A scratch wound healing assay and the expression of matrix metalloproteinases MMPs and a tanswell assay were used to investigate cell migration and invasion respectively Additionally we uncovered upstream signaling Akt/mTOR and p38 pathways induced by incubation U87 and U251 cell lines with fucoxanthin that mediated cell apoptosis migration and invasion by using PI3K and p38 inhibitors Moreover incubation of fucoxanthin obviously reduced the weight and volume of glioma mass of U87 cells in nude mice Furthermore we also examined the glioma mass of U87 cells by hematoxylineosin staining TUNEL assay and western blot and these outcomes in vivo consistently confirmed that above results in vitro Taken together these findings suggest that fucoxanthin augments apoptosis and reduces cell proliferation migration and invasion and reveals a potential mechanism of fucoxanthinmediated Akt/mTOR and p38 susspression in human glioblastoma cell line
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