Authors: Bo Xiao Huazhen Liu Zeyun Gu Sining Liu Cheng Ji
Publish Date: 2015/09/28
Volume: 40, Issue: 11, Pages: 2348-2356
Abstract
Cell transplantation of neural stem cells NSCs is a promising approach for neurological recovery both structurally and functionally However one big obstacle is to promote differentiation of NSCs into neurons and the followed maturation In the present study we aimed to investigate the protective effect of taurine on the differentiation of NSCs and subsequent maturation of their neuronal lineage when exposed to oxygen–glucose deprivation OGD The results suggested that taurine 5–20 mM promoted the viability and proliferation of NSCs and it protected against 8 h of OGD induced impairments Furthermore 20 mM taurine promoted NSCs to differentiate into neurons after 7 days of culture and it also protected against the suppressive impairments of 8 h of OGD Consistently taurine 20 mM promoted the neurite sprouting and outgrowth of the NSC differentiated neurons after 14 days of differentiation which were significantly inhibited by OGD 8 h At D21 the mushroom spines and spine density were promoted or restored by 20 mM taurine Taken together the enhanced viability and proliferation of NSCs more differentiated neurons and the promoted maturation of neurons by 20 mM taurine support its therapeutic application during stem cell therapy to enhance neurological recovery Moreover it protected against the impairments induced by OGD which may highlight its role for a more direct therapeutic application especially in an ischemic stroke environment
Keywords: