Authors: Berislav Bošnjak Vanesa Ivetić Tkalčević Koraljka Đurić Daniela Belamarić Snježana Čužić Željko Ferenčić Karmen Brajša Ines Glojnarić Roberto Antolović Boška Hrvačić
Publish Date: 2009/05/08
Volume: 58, Issue: 11, Pages: 773-
Abstract
To investigate whether challenge with increasing allergen doses could differently affect allergeninduced airway hyperresponsiveness AHR and inflammatory cell accumulation in mouse model of asthma providing an experimental model to investigate their relationshipBoth AHR and inflammatory cell accumulation were proportional to the ovalbumin dose used for challenge However in group challenged with 10 μg of ovalbumin airway inflammation was present although allergeninduced AHR was not detected Additional analysis indicated that neither mucous hyperproduction nor eosinophil degranulation could be correlated to presence of AHR in this model whereas concentration of interleukin IL13 in BALF was increased only in those groups in which AHR was presentAltogether intranasal challenge of mice with increasing allergen doses could serve as a suitable experimental system for investigation of mechanisms by which airway inflammation leads to allergeninduced AHR Our initial findings are in line with previous reports that dissociate AHR from amount of eosinophil accumulation and imply the role of IL13 in this processThis work was supported by GlaxoSmithKline Research Centre Zagreb Limited Zagreb Croatia The authors wish to acknowledge the excellent technical help of Ms M Horvatinčić I Novaković R Povrženić S Skender M Škalic and Mr V Vrban The authors would also like to thank Drs Alenka Gagro and Michael J Parnham for insightful comments during manuscript preparation
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