Authors: Miriam H P van Lieshout Tom van der Poll Cornelis van’t Veer
Publish Date: 2014/08/15
Volume: 63, Issue: 11, Pages: 927-933
Abstract
Mice were infected with different doses ~2 × 104–2 × 106 CFU of E coli O18K1 and treated after 8 h with ceftriaxone 20 mg/kg ip combined with either E5564 10 mg/kg iv or vehicle For survival studies this treatment was repeated every 12 h Bacterial loads and inflammatory parameters were determined after 20 h in peritoneal lavage fluid blood liver and lung tissue Plasma creatinin AST ALT and LDH were determined to assess organ injuryE5564 impaired bacterial clearance under the antibiotic regime after infection with a low dose E coli 17 × 104 CFU while renal function was slightly preserved No differences were observed in bacterial load and organ damage after infection with a tenfold higher 17 × 105 E coli bacterial dose While treatment with E5564 slightly attenuated inflammatory markers provoked by the sublethal doses of 104–105 E coli under the antibiotic regime it did not affect lethality evoked by infection with 17 × 106 E coli
Keywords: