Authors: B O Nilsson I Kockum E Rosengren
Publish Date: 2014/02/28
Volume: 48, Issue: 4, Pages: 176-180
Abstract
Objective To determine if putrescine and the higher polyamines spermidine and spermine are released from cultured vascular smooth muscle cells¶Material Vascular smooth muscle cell line A7r5¶Treatment Cells were treated with aminoguanidine 10 or 100 μM for 1 to 24 h with or without fetal calf serum 10 present in the culture medium¶Methods Cellular and medium concentrations of polyamines were determined by liquid chromatography Total cellular protein was determined by the Bradford procedure Students twotailed ttest was used for statistical calculations¶Results A constitutive release of putrescine was disclosed within 5 h if serum diamine oxidase was inhibited by 10 μM aminoguanidine The release was linear with time for 24 h and specific for putrescine in the sense that the higher polyamines spermidine and spermine despite similar cellular concentrations were not released Similar amounts of putrescine were released from the smooth muscle cells whether or not culture medium contained serum Cells that had been cultured in medium from which fetal calf serum had been omitted for last 48 h contained less putrescine spermidine and protein than those that persisted in medium that contained serum¶Conclusion A constitutive putrescine release from vascular smooth muscle cells is disclosed in the presence of aminoguanidine
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