Authors: Huma Jawed Shazia Anjum Shahid I Awan Shabana U Simjee
Publish Date: 2011/08/27
Volume: 60, Issue: 12, Pages: 1113-1120
Abstract
Glucosamine is a naturally occurring amino monosaccharide that maintains the elasticity and strength of the cartilage tissues It has been used to treat osteoarthritis in humans however in severe conditions of inflammation and pain glucosamine alone is not enough and it is important to improve its biological activity Our research group has recently taken an interest in the synthetic manipulation of amino sugars to develop some efficient pharmacophores eg βdglucosamine to combat rheumatoid arthritis and tested its antiarthritic effects in the collageninduced arthritis CIA model in ratsArthritis was induced in female Sprague–Dawley rats by multiple intradermal injections of bovine type II collagen and challenged again with the same antigen preparation 7 days later Arthritis was evaluated by arthritic score body weight loss paw volume measurement and histological changesThe animals in the arthritic control group showed a gradual decrease in their body weight and concurrent increase in the paw volumes compared to the normal control group In contrast increased hind paw swelling was significantly suppressed with no further noticeable reduction in body weight in the glucosamine p 005 and GN1treated p 002 arthritic animals Histopathological evaluation of isolated knee joints by grading system and classification of the stages in arthritic lesion development revealed suppression of the inflammatory changes in the GN1treated animals Moreover both the proinflammatory markers Creactive protein CRP and lowdensity lipoprotein LDL levels were found to be significantly decreased in animals treated with GN1 p 003 for CRP and p 005 for LDL compared to the arthritic control group
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