Authors: James David Shutt Philip Boger James Richard Neale Praful Patel Anthony Peter Sampson
Publish Date: 2012/08/01
Volume: 61, Issue: 12, Pages: 1379-1384
Abstract
Leukotriene LT B4 is a lipid inflammatory mediator implicated in tumorigenesis in animal models of Barrett’s oesophagitis but little is known about the cysteinylleukotrienes LTC4 LTD4 LTE4 which have distinct inflammatory and tumorigenic actions in other tissues We recently showed that the terminal enzymes for the synthesis of both LT families are highly expressed in human oesophageal adenocarcinoma OA tissues This study therefore examined the capacity of Barrett’s metaplasia BM and OA tissues to synthesise LTs in vitroOesophageal biopsies from patients with BM n = 14 highgrade dysplasia n = 2 OA n = 11 and squamous control tissues n = 11 were cultured with calcium ionophore A32187 2 μM for 60 min LTB4 and cysteinylleukotrienes were extracted and measured by specific enzyme immunoassaysLevels of LTB4 and cysteinylleukotrienes were 86fold P 001 and 24fold P 002 higher respectively in OA tissues than in squamous control tissues but levels in BM tissues n = 14 were not altered Production of the two LT families correlated across all tissue types r = 062 p 000005
Keywords: