Authors: Yang Li Thanh Truc Weimin Wang
Publish Date: 2009/12/20
Volume: 2, Issue: 1, Pages: 43-46
Abstract
Thirteen polymorphic microsatellite loci from topmouth culter Culter alburnus were isolated using the fast isolation by AFLP of sequences containing repeats FIASCO protocol These loci were characterized by genotyping 40 individuals The observed number of alleles ranged from 7 to 15 with an average of 107 of each loci The expected and observed heterozygosities ranged from 07047 to 09193 and from 04500 to 08250 respectively Among these polymorphic microsatellite loci three of the loci TC04 TC07 and TC12 significantly deviated from P 005 Hardy–Weinberg Equilibrium after sequential Bonferroni correction All of the microsatellite loci were in linkage equilibrium These microsatellite markers would be useful for investigating the genetic diversity and population structure of topmouth culter
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