Twentynine microsatellite markers for two Austral

Authors: Erica Todd David Blair Mark Hamann Dean Jerry

Publish Date: 2011/01/05

Volume: 3, Issue: 3, Pages: 449-456

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Abstract

454 shotgun sequencing was used to identify thousands of in silico microsatellite loci from two Australian freshwater turtle species Pleurodira Chelidae Elseya albagula and Emydura macquarii krefftii Twentynine polymorphic microsatellites were subsequently developed and tested as markers 15 for E m krefftii and 14 for E albagula The former marker set showed greater allelic richness mean N A  = 77 range 3–15 than the latter mean N A  = 44 range 2–8 Levels of expected heterozygosity were high for both marker suites mean H E  = 0718 range 0177–0875 and mean H E  = 0567 range 0317–0827 respectively indicating both are suitable for studies of population genetic structure connectivity and mating systems in these taxa Crossspecies amplification in 12 other shortnecked freshwater turtle taxa highlighted the potential utility of these markers in other species notably Emydura spp Rheodytes leukops and Elusor macrurusWe thank Queensland Turtle Research and Jason Schaffer for providing tissue samples and fieldwork opportunities Arthur Georges kindly provided many tissue samples for crossspecies testing Kyall Zenger provided helpful sequencing advice and assistance Ivan Lawler Dave Jones and Carolyn SmithKeune also provided invaluable advice and support This work was partially funded by research grants from the Wildlife Preservation Society of Queensland Wildlife Preservation Society of Australia and the Peter Rankin Trust Fund for Herpetology Research was conducted under ethics permit no A1444

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