Journal Title
Title of Journal: Conservation Genet Resour
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Abbravation: Conservation Genetics Resources
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Publisher
Springer Netherlands
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Authors: Julie A Jedlicka Abhineet M Sharma Rodrigo P P Almeida
Publish Date: 2013/03/10
Volume: 5, Issue: 3, Pages: 879-885
Abstract
The emerging field of molecular scatology enables critical testing of food web theory The noninvasive application of molecular tools allows for sequencing of prey DNA from predator fecal matter evaluating diet breadth and foraging guild While insectivorous bats are obscure foragers compared to most insectivorous birds more is known about which arthropod species bats consume because molecular techniques have been optimized for mammalian systems not avian physiology Our research objective was to use molecular tools to detect arthropod prey in the fecal matter of an insectivorous avian predator We used Western Bluebird Sialia mexicana as a model predator due to its generalist foraging strategy We compared two fecal DNA extraction kits 1 Qiagen’s DNA stool mini kits used widely in dietary studies on bats and 2 Zymo’s Soil/Fecal DNA MiniPrep kits not currently cited in the molecular scatology literature We successfully extracted DNA only with the Zymo kit amplified mitochondrial cytochrome oxidase c subunit I genes sequenced and identified the arthropod prey A spiked PCR experiment showed evidence of possible inhibitors remaining in the Qiagen kit extractions Overall arthropod prey from seven different orders and five different classes were identified We discuss the ecological implications of these data and suggest areas of future research applying molecular techniques to avian fecal matter Consistent methodological advancement will enable molecular scatology to identify ecosystem services provided by insectivorous birds develop ecological theory and inform predator conservation effortsNational Science Foundation DBI1103583 and the University of California Agricultural Experiment Station provided funding for this project We thank Ron Rosenbrand Diane Kenworthy Robert Burney Joel Peterson Bedrock Vineyards and Spring Mountain Vineyards for access to bluebird nest boxes We thank Marie Lefebvre for customizing a Perl script to obtain BLAST identifications for sequences Four anonymous reviewers provided helpful comments on earlier versions of this manuscript
Keywords:
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- Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
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- Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
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- Review of the Dra I COI - COII test for the conservation of the black honeybee ( Apis mellifera mellifera )
- Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace ( Tribolodon hakonensis )
- Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli
- Isolation of polymorphic microsatellite markers from amphioxus ( Branchiostoma belcheri ) and a test of cross-species amplification
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