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Title of Journal: Conservation Genet Resour

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Abbravation: Conservation Genetics Resources

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Springer Netherlands

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1877-7260

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Development of 16 microsatellite loci for the enda

Authors: Amanda L McLean Steven J B Cooper Melanie L Lancaster Susan M Carthew
Publish Date: 2013/12/04
Volume: 6, Issue: 2, Pages: 323-326
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Abstract

Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach and three loci developed for Sminthopsis douglasi were optimized for the endangered sandhill dunnart Sminthopsis psammophila Nineteen loci 16 S psammophila and three S douglasi were tested on 38 individual S psammophila from two populations in South Australia Three loci deviated from Hardy–Weinberg equilibrium possibly due to null alleles No evidence of linkage disequilibrium was detected We recommend a final panel of 17 loci for future genetic analyses of S psammophila which will aid in conservation management of this poorly understood and nationally endangered marsupialFunding was obtained from OneSteel Whyalla Nature Foundation SA Holswoth Wildlife Research Endowment Australian and Pacific Science Foundation and Field Naturalists Society of South Australia We thank the Department of Environment Water and Natural Resources for access to Ironstone Hill Conservation Park Matthew Ward DEWNR for collecting tissue samples from Yellabinna Regional Reserve and Kathy Saint for laboratory assistance

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  2. Polymorphic microsatellite loci in the Chinese skink, Plestiodon chinensis (squamata: scincidae)
  3. Development and application of a molecular sexing protocol in the climate change-sensitive American pika
  4. Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
  5. Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan
  6. Isolation and characterization of 16 microsatellite markers in Intsia palembanica , a high-value tropical hardwood species
  7. Fecal samples fail in PCR-based diagnosis of malaria parasite infection in birds
  8. Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
  9. The development of 24 polymorphic microsatellite loci for the endangered barred galaxias, Galaxias fuscus , through next generation DNA sequencing
  10. Isolation and characterization of novel microsatellite markers in Gekko gecko (Reptilia: Gekkonidae)
  11. Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii : development from 454-sequence data and utility in related taxa
  12. Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon
  13. Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)
  14. Development of microsatellite markers for the spiny-bellied frog Quasipaa boulengeri (Anura: Dicroglossidae) through transcriptome sequencing
  15. Development of nine polymorphic microsatellite loci in the squirrel glider ( Petaurus norfolcensis )
  16. Evaluation of microsatellites for common ungulates in the South African wildlife industry
  17. Nine microsatellite loci for the Malagasy Poison Frogs ( Mantella )
  18. Characterization of the complete chloroplast genome of Elaeagnus mollis , a rare and endangered oil plant
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