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Title of Journal: Conservation Genet Resour

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Abbravation: Conservation Genetics Resources

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Springer Netherlands

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DOI

10.1007/s00120-009-2106-1

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ISSN

1877-7260

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Isolation and characterization of novel microsatel

Authors: Saifei Gao Dongmei Yu Jianjun Peng Ying Wang Meilan Fu Xiqing Liu Shijia Hu Lili Wang Lingfu Gao
Publish Date: 2011/01/15
Volume: 3, Issue: 3, Pages: 467-469
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Abstract

Eighteen novel microsatellite loci were isolated from Gekko gecko with a partial genomic library enriched for tandem repeat sequences of CA12 Polymorphism and genetic evaluation were investigated for these markers in 22 wild G gecko individuals collected from Guangzhou Forest Police Bureau of China Seventeen polymorphic loci were observed and the allele number ranged from 5 to 10 per locus with the average of 79 The observed and expected heterozygosity varied from 04500 to 06818 and 07812 to 09088 respectively The average polymorphism information content of this population was 08265 which showed that the wild G gecko population had a high level of genetic variability Only two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction No significant linkage disequilibrium was detected These microsatellite loci will be useful for population genetics ecology and determining effective conservation measures of wild G geckoWe acknowledge the financial support of the Natural Scientific Foundation of Guangdong Province No 9151026001000003 Excellent Young Scientist Foundation of Guangdong Academy of Sciences No 200804 Guangdong Provincial Public Laboratory on Wild Animal Conservation and Management No 200901


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Other Papers In This Journal:

  1. Development of 44 gene-based SNP markers in Zhikong scallop, Chlamys farreri
  2. Polymorphic microsatellite loci in the Chinese skink, Plestiodon chinensis (squamata: scincidae)
  3. Development and application of a molecular sexing protocol in the climate change-sensitive American pika
  4. Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
  5. Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan
  6. Isolation and characterization of 16 microsatellite markers in Intsia palembanica , a high-value tropical hardwood species
  7. Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
  8. Fecal samples fail in PCR-based diagnosis of malaria parasite infection in birds
  9. Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
  10. The development of 24 polymorphic microsatellite loci for the endangered barred galaxias, Galaxias fuscus , through next generation DNA sequencing
  11. Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii : development from 454-sequence data and utility in related taxa
  12. Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon
  13. Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)
  14. Development of microsatellite markers for the spiny-bellied frog Quasipaa boulengeri (Anura: Dicroglossidae) through transcriptome sequencing
  15. Development of nine polymorphic microsatellite loci in the squirrel glider ( Petaurus norfolcensis )
  16. Evaluation of microsatellites for common ungulates in the South African wildlife industry
  17. Nine microsatellite loci for the Malagasy Poison Frogs ( Mantella )
  18. Characterization of the complete chloroplast genome of Elaeagnus mollis , a rare and endangered oil plant
  19. How to use SNPs and other diagnostic diallelic genetic markers to identify the species composition of multi-species hybrids
  20. A rapid method for species identification and interspecies introgression based on a PCR–RFLP: application to the closely related Neotropical toads Rhinella marina and R. schneideri (Anura: Bufonidae)
  21. Development of 13 polymorphic microsatellite loci for the Greenland Halibut ( Reinhardtius hippoglossoides )
  22. Environmental DNA detection of redfin perch, Perca fluviatilis
  23. Development of diploid microsatellite markers for the North American paddlefish ( Polyodon spathula )
  24. Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps
  25. Development and characterization of twelve polymorphic microsatellite loci for the Chinese concave-eared frog (Odorrana tormota)
  26. Characterization of ten novel microsatellite markers in Awaous guamensis with comments on cross amplification in congeners and other amphidromous fish native to Hawai’i
  27. Development and characterization of new microsatellite markers for Amur sturgeon ( Acipenser schrenckii)
  28. A species-specific microsatellite marker to discriminate blue catfish, channel catfish and F1 hybrids
  29. Eighteen microsatellite loci for the crown-of-thorns starfish, Acanthaster planci
  30. Development and characterization of polymorphic microsatellite marker for East Asian species of the genus Plestiodon
  31. Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes
  32. Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies
  33. Twenty-nine polymorphic microsatellite loci in Cyclura carinata , the Turks and Caicos Iguana, a critically endangered island endemic
  34. Development of polymorphic microsatellite markers in topmouth culter ( Culter alburnus )
  35. Review of the Dra I COI - COII test for the conservation of the black honeybee ( Apis mellifera mellifera )
  36. Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace ( Tribolodon hakonensis )
  37. Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli
  38. Isolation of polymorphic microsatellite markers from amphioxus ( Branchiostoma belcheri ) and a test of cross-species amplification
  39. Molecular tools reveal diets of insectivorous birds from predator fecal matter

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