Evaluation of microsatellites for common ungulates

Authors: Susan M Miller Amy B Clarke Paulette Bloomer Alan J Guthrie Cindy K Harper

Publish Date: 2016/06/01

Volume: 8, Issue: 3, Pages: 329-341

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Abstract

Wildlife is a multimillion dollar industry in South Africa ranging from intensively farmed animals on small properties to freeroaming animals in large functional ecosystems Specific concerns surrounding the conservation value of the intensively managed wildlife have been raised Increasingly it is recognised that genetics must be considered when developing conservation policy and management practices Minimal genetic data are available to assist with decisions within the industry Microsatellite markers have been developed for some species and are currently used for individual identification parentage assessment and to inform translocation decisions However validation for standardized application in wildlife management has not been completed Common species for which conservation management decisions have to be made include Cape buffalo Syncerus caffer blue wildebeest Connochaetes taurinus taurinus common impala Aepyceros melampus and sable antelope Hippotragus niger Using existing microsatellite markers for domestic and wild species we have developed multiplex panels for use in these species We discriminated between 751 buffalo four pairs were not distinguishable 1307 blue wildebeest three pairs were not distinguishable 580 impala and 493 sable antelope We determined parentage in four case studies using a combination of microsatellite and demographic data We also tested microsatellite markers on roan antelope Hippotragus equinus gemsbok Oryx gazella and eland Tragelaphus oryx and report preliminary data Further research to improve this validation includes extensive sampling from more diverse areas targeted or full genome sequencing to identify additional microsatellite loci development of sampling kits and training of veterinarians as well as expansion of the species tested

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