Journal Title
Title of Journal: Conservation Genet Resour
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Abbravation: Conservation Genetics Resources
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Publisher
Springer Netherlands
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Authors: Adam D Miller Anthony van Rooyen Renae M Ayres Tarmo A Raadik Peter Fairbrother Andrew R Weeks
Publish Date: 2012/02/28
Volume: 4, Issue: 3, Pages: 613-616
Abstract
Barred galaxias Galaxias fuscus is an endangered freshwater fish confined to headwaters of mountain streams in the Goulburn River system in central Victoria Australia Next generation DNA sequencing was used to develop a suite of microsatellite markers that can be used for future population genetic assessments of G fuscus A total of 24 polymorphic loci were identified and 12 characterized using 30 individuals from a single sample locale We observed low to moderate genetic variation across most loci mean number of alleles per locus = 258 mean expected heterozygosity = 037 with no evidence of individual loci deviating significantly from Hardy–Weinberg equilibrium Marker independence was confirmed with tests for linkage disequilibrium and analyses indicated no evidence of null alleles across loci The 12 markers characterized in the present study provide a valuable resource for future population genetic assessments and management of G fuscus in AustraliaThe authors thank Michael Nicol Dean Hartwell and Graeme Seppings for help with sample collection This project was funded through the Commonwealth Caring for Our Country program and the Victorian and Commonwealth government’s Statewide Bushfire Recovery Plan and was conducted under the Fisheries Act 1995 permit RP827 National Parks Act 1975 and Flora and Fauna Guarantee Act 1988 permit 10005451 and animal ethics permit AEC07/24
Keywords:
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- Polymorphic microsatellite loci in the Chinese skink, Plestiodon chinensis (squamata: scincidae)
- Development and application of a molecular sexing protocol in the climate change-sensitive American pika
- Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
- Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan
- Isolation and characterization of 16 microsatellite markers in Intsia palembanica , a high-value tropical hardwood species
- Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
- Fecal samples fail in PCR-based diagnosis of malaria parasite infection in birds
- Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
- Isolation and characterization of novel microsatellite markers in Gekko gecko (Reptilia: Gekkonidae)
- Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii : development from 454-sequence data and utility in related taxa
- Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon
- Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)
- Development of microsatellite markers for the spiny-bellied frog Quasipaa boulengeri (Anura: Dicroglossidae) through transcriptome sequencing
- Development of nine polymorphic microsatellite loci in the squirrel glider ( Petaurus norfolcensis )
- Evaluation of microsatellites for common ungulates in the South African wildlife industry
- Nine microsatellite loci for the Malagasy Poison Frogs ( Mantella )
- Characterization of the complete chloroplast genome of Elaeagnus mollis , a rare and endangered oil plant
- How to use SNPs and other diagnostic diallelic genetic markers to identify the species composition of multi-species hybrids
- A rapid method for species identification and interspecies introgression based on a PCR–RFLP: application to the closely related Neotropical toads Rhinella marina and R. schneideri (Anura: Bufonidae)
- Development of 13 polymorphic microsatellite loci for the Greenland Halibut ( Reinhardtius hippoglossoides )
- Environmental DNA detection of redfin perch, Perca fluviatilis
- Development of diploid microsatellite markers for the North American paddlefish ( Polyodon spathula )
- Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps
- Development and characterization of twelve polymorphic microsatellite loci for the Chinese concave-eared frog (Odorrana tormota)
- Characterization of ten novel microsatellite markers in Awaous guamensis with comments on cross amplification in congeners and other amphidromous fish native to Hawai’i
- Development and characterization of new microsatellite markers for Amur sturgeon ( Acipenser schrenckii)
- A species-specific microsatellite marker to discriminate blue catfish, channel catfish and F1 hybrids
- Eighteen microsatellite loci for the crown-of-thorns starfish, Acanthaster planci
- Development and characterization of polymorphic microsatellite marker for East Asian species of the genus Plestiodon
- Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes
- Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies
- Twenty-nine polymorphic microsatellite loci in Cyclura carinata , the Turks and Caicos Iguana, a critically endangered island endemic
- Development of polymorphic microsatellite markers in topmouth culter ( Culter alburnus )
- Review of the Dra I COI - COII test for the conservation of the black honeybee ( Apis mellifera mellifera )
- Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace ( Tribolodon hakonensis )
- Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli
- Isolation of polymorphic microsatellite markers from amphioxus ( Branchiostoma belcheri ) and a test of cross-species amplification
- Molecular tools reveal diets of insectivorous birds from predator fecal matter
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