Authors: Siqi Yuan Yun Xia Yuchi Zheng Xiaomao Zeng
Publish Date: 2014/10/10
Volume: 7, Issue: 1, Pages: 229-231
Abstract
We detected and isolated thirtytwo microsatellite loci from transcriptomederived microsatellite DNA for Quasipaa boulengeri The isolated loci were all polymorphic and the number of alleles per locus ranged from 2 to 10 The observed and expected heterozygosity per locus varied from 01493 to 09375 and from 01563 to 09688 respectively By multiple comparisons with Bonferroni correction seven loci were deviated from Hardy–Weinberg equilibrium and significant linkage disequilibrium was detected among twentyfive pairs of loci Those variable microsatellite sites will be applied to assess gene flow between populations and investigate the evolutionary process of chromosomal polymorphism Moreover microsatellite loci were served as a valuable resource for drawing up criterion for the conservation management of this threatened species
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